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It has been shown that the fluorescence quantum yield of ANS increases strongly with decreasing temperature and that the position of the fluorescence bond shifts towards the blue. Figure 17 shows emission curves obtained from micellar solutions in A O T within the +50 to -3OOC temperature range (39). An increase in q5F has been attributed to a decreased rate constant for the nonradiative deactivation process of ANS singlet excited state, as the temperature coefficient of the radiative rate constant is relatively small.

However, the water-in-oil emulsion procedure is very far from being problem-free and lacks the facility of the mixed-solvent technique. The turbidity of the emulsions precludes analytical techniques that rely on optical transparency. However, recent exploratory studies using EPR on supercooled emulsions of hemoproteins show promise (F. Franks, unpublished data), and, in principle, the same should apply to NMR-based techniques. T h e emulsion method cannot at present be applied to reactions that proceed rapidly at 0°C or in conjunction with proteins of membrane origin that are likely to be adsorbed at the oil-water interface.

T h e effect of added solutes on the solubilization of water in nonaqueous solutions involving Aerosol OT (AOT) has been treated in 56 PIERRE DOUZOU only a few papers (58-60) and changes in the minimum temperature of solubilization, as well as region ranges, have been measured. It has been found that the precipitate was composed of surfactant and water. Most investigations of this kind have been carried out at temperatures above OOC, while it is known that many formulae of micromicellar solutions retain homogeneity and transparency below 0°C.

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